StepOne is a program for Windows that gives access to advanced real-time PCR technology to a wide range of users. The system is built to measure amplification as it occurs, cycle by cycle, allowing for precise and quantitative data gathering. Alternatively, you may use a similar application called Bio Rad CFX Manager.

Operation

Starting at the homepage, you are able to navigate through all aspects of the real-time PCR process including sample and reaction set-up, thermal cycling and fluorescent detection. The provided tools enable you to analyze and interpret experimentation results. Depending on the project design, the system can help you select and order PCR reagents online by means of links.

Design wizard

What is more, the software offers a design wizard to assist you in setting up your first experiment. All you need to do is click on the corresponding button to launch it and start answering questions about the tasks you want to perform. The wizard walks you through quantitation methods, detection chemistry, ramp speeds and nucleic acid template styles. It guides the entire process, including reaction mix and standard dilution calculations.

Features

free to download and use;
compatible with modern Windows versions;
gives you the ability to perform different PCR experiments;
allows you to analyze and interpret results.
it is possible to order necessary reagents online;
comes with a dedicated project wizard.