LightCycler is a Windows application that allows molecular biologists to simplify a wide range of PCR operations. It employs advanced algorithms to identify optimal primer sequences and probe placements, ensuring high specificity and sensitivity. Please note that LightCycler is a paid program. There is no free trial version.

Assay design and optimization

Using this utility you can reduce the time and effort required to obtain accurate PCR test results. Instruments for automatically optimizing various operation parameters such as the cycle number, annealing temperature and magnesium level are provided.

It is possible to capture input fluorescence data, allowing for the quantification of target DNA or RNA concentrations. Moreover, there are tools for generating standard and melting curves as well as for differential gene expression analysis.

Other apps for working with genetic sequences like GeneMapper are available for download.

Multiplexing

Users can detect several targets in a single PCR reaction. This functionality is particularly helpful for processing complex samples or for conducting advanced pathogen detection.

Features

requires a license to download and use;
contains tools for automating the process of real time polymerase chain reaction analysis;
you can capture fluorescence data to quantify target DNA and RNA concentrations;
it is possible to conduct pathogen detection operations;
compatible with modern versions of Windows.